Methods: Standard disc diffusion techniques were employed to test seven antibiotics (Rifampicin, Clindamycin, Levofloxacin, Meropenem, Ampicillin, Gentamicin, and Tetracycline) against the three bacterial species. Sensitivity and resistance profiles were analyzed to determine the most effective therapeutic agents.

Results: Meropenem and Gentamicin exhibited the highest overall efficacy, each achieving a sensitivity rate of 90.9%, followed by Levofloxacin (81.8%). Ampicillin also demonstrated moderate sensitivity across isolates. Conversely, high resistance rates were observed for Rifampicin (72.7%), Tetracycline (72.7%), and Clindamycin, particularly among Gram-negative bacteria. Notably, S. aureus displayed greater overall susceptibility compared to P. aeruginosa and E. coli.

Conclusion: Meropenem, Gentamicin, and Levofloxacin were the most effective antibiotics against the studied isolates. However, the substantial resistance to several first-line agents emphasizes the urgent need for antimicrobial stewardship and continuous local resistance monitoring. Developing antibiotic policies tailored to regional susceptibility patterns is crucial to optimize treatment outcomes and mitigate the spread of multidrug-resistant organisms.